Satellite Workshop - Advancing Correlative Light and Electron Cryo-Microscopy: Bridging Molecular Precision and Tissue Architecture

Registration:

There is no registration fee, but please register here for the event:
https://indico.desy.de/event/48246/

Date: May 6, 2025

Confirmed speakers:

Karolinska Institutet
Human Technopole
MPI Biochemistry
MPI Molecular Physiology
University of Basel

Sponsored Talks:

Thermo Fisher Scientific

Organizers:

Carolin Seuring (Universität Hamburg, CSSB Hamburg)
Ulrike Laugks (Universität Hamburg, CSSB Hamburg)
Kay Grünewald (LIV, Universität Hamburg, CSSB Hamburg)
Cornelia Cazey (Universität Hamburg, CSSB Hamburg)

Programme:

Schedule
11:30 - 12:30
Early Arrivals, Poster Session & Industry Exhibits

• Meet the exhibitors: Thermo Fisher Scientific, Delmic, TESCAN, DENSsolutions

• Poster viewing & networking for early participants
12:30 - 12:55
Registration & Light Welcome Lunch
12:55 - 13:00
Opening Remarks
• Kay Grünewald (CSSB)
13:00 - 13:20
Espresso Talks
• 1 slide per participant – quick intros to foster networking and discussion
13:20 - 15:00
Advances in Cryo-ET workflows and targeted FIB milling

• 13:20 - 13:40 | An improved cryo-FIB-ET workflow towards quantitative cryo-electron tomography – Sebastian Tacke (MPI Molecular Physiology, Dortmund)

• 13:40 - 14:00 | Targeting and Sample Preparation of Large Volume Samples Using FIB-SEM-integrated Light Microscopes and Serial Lift-Out – Oda Schiøtz (MPI Biochemistry, Martinsried)

• 14:00 - 14:20 | Lift-Out-Towards a Biopsy at the Nanoscale – Philip Erdmann (Technopol)

• 14:20 - 14:40 | Silicon-Based Sample Carrier for Cryogenic Electron Microscopy - Vasilis Papadimitriou (DENSsolutions)

• 14:40 - 15:00 | Advances in Integrated Fluorescence Microscopy for Cryo-FIB Microscopes – Radovan Spurny (Thermofisher)
15:00 - 15:30
Coffee Break & Networking
15:30 - 16:30
Challenges in Correlative Imaging and Data Integration

• 15:30 - 15:50 | Cryo-CLEM studies of neurons – Ruben Fernandez Busnadiego (University of Göttingen)

• 15:50 - 16:10 | Addressing population heterogeneity in photosynthesis with Spectrally Resolved CLEM – Sofie van Dorst (University of Basel)

• 16:10 - 16:30 | Addressing Two Challenges in CLEM: Ice Contamination and Sample Charging – Amy Bondy (Karolinska Institutet)
16:30 - 16:50
Coffee Break & Networking
16:50 - 17:30
Industry Insights and Future Directions

• 16:50 - 17:10 | Latest developments in precise correlative cryo-FIB milling for cryo-ET lamella production – Marit Smeets (Delmic)

• 17:10 - 17:30 | Cryo Microscopy Brew: A Smooth Blend of Light, Electron, and Soft X-ray for Targeting Unlabeled Features – Martina Zánová (TESCAN)

• 17:30 - 17:40 | Future Directions - Challenges in Super-resolution imaging – Julian Falckenhayn (CSSB)
17:40 - 18:30
Panel Discussion: Future Perspectives of Cryo-CLEM in Structural Biology
18:30 - 20:00
Networking Reception and Drinks in CSSB Foyer: Summary & Closing Remarks

Poster:

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Sponsors:

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